mouse monoclonal antibody against cd31  (Proteintech)

Journal: PLOS ONE

Article Title: Editorial Note: Olmesartan Potentiates the Anti-Angiogenic Effect of Sorafenib in Mice Bearing Ehrlich’s Ascites Carcinoma: Role of Angiotensin (1–7)

doi: 10.1371/journal.pone.0316407

Figure Lengend Snippet: Antibody details.

Article Snippet: CD31 , Mouse monoclonal antibody against human CD31 , Thermo Fisher Scientific (Fremont, USA). , #MS-353-R7.

Techniques:

Journal: Nature Communications

Article Title: NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice

doi: 10.1038/s41467-024-54407-7

Figure Lengend Snippet: A Left: representative immunofluorescence confocal microscopy showing the endothelial cell marker CD31 (red) and the mesenchymal cell marker α smooth muscle actin (α-SMA) (green) in human umbilical vein endothelial cells (HUVEC) treated with cytokines (10 mg/μL transforming growth factor β [TGF-β1] and 1 ng/μL interleukin-1β [IL-1β]), expressing exogenous NOTCH1 intracellular domain (NICD1), or mitoNICD1 in low-power field (LPF) (10×) (top, scale bar=200 μm) and high-power field (HPF) (×40) (bottom, scale bar = 50 μm)). Nuclei are stained with DAPI (blue). Right: Quantification of α-SMA positive (α-SMA + ) cells per LPF ( n = 5 biological samples per group). B Representative western blot illustrating endothelial cell markers, including CD31, vascular endothelial cadherin (VE-Cad), and vascular endothelial growth factor receptor (VEGFR), and mesenchymal cell markers, including α-SMA, N-Cadherin (N-Cad), Vimentin, and Actin, in HUVEC expressing exogenous NICD1, mitoNICD1, or not. C Representative western blot illustrating the phosphorylation of pyruvate dehydrogenase E1 subunit alpha 1 (PDHA1) (p-PDHA1) at serine 293 (Ser293) in HUVEC treated with PDH-activator dichloroacetate (DCA) (10 mmol/L), expressing mitoNICD1, or expressing mitoNICD1 and supplemented with PDH inhibitor PS-48 (100 μmol/L) as well. D Top: profile of oxygen consumption rate (OCR) of HUVEC treated with DCA (10 mmol/L), expressing mitoNICD1, or expressing mitoNICD1 and supplemented with PS-48 (100 μmol/L) as well. Bottom: Quantification of basal respiration and maximal respiration indicated by OCR ( n = 3 biological samples per group). E Left: representative immunofluorescence confocal microscopy showing CD31 (red) and α-SMA (green) in HUVEC treated with DCA (10 mmol/L), expressing mitoNICD1, or expressing mitoNICD1 and supplemented with PS-48 (100 μmol/L) as well (Scale bar=200 μm). Right: quantification of α-SMA + cells per LPF ( n = 5 biological samples per group). F Representative immunofluorescence confocal microscopy of CD31 (red) and α-SMA (green) in HUVEC treated with 5, 10, or 20 mmol/L DCA (Scale bar = 200 μm). Right: quantification of α-SMA + cells per LPF ( n = 5 biological samples per group). Source data are provided as a Source Data file. For A , D – F , data are presented as mean values ± SD; P values were calculated by unpaired Student’s t test with two-tailed analysis without adjustments.

Article Snippet: To assess the expression of CD31 and α-SMA in EndMT assays, fixed and blocked HUVEC cells were stained with rabbit polyclonal antibody against α-SMA (1:200) and mouse monoclonal antibody against CD31 (1:200, #3528, Proteintech) overnight and incubated with Alexa 488-conjugated goat anti-rabbit antibody and Alexa 594-conjugated goat anti-mouse antibody (A11032, Invitrogen) at 1:1,000, following the abovementioned procedures.

Techniques: Immunofluorescence, Confocal Microscopy, Marker, Expressing, Staining, Western Blot, Phospho-proteomics, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Nlrp3 Deficiency Does Not Substantially Affect Femoral Fracture Healing in Mice

doi: 10.3390/ijms252111788

Figure Lengend Snippet: ( a – d ) Representative histological images of TRAP-positive osteoclasts (arrowheads) within callus tissue of wildtype ( a , b ) and Nlrp3 −/− mice ( c , d ) at 2 ( a , c ) and 5 weeks ( b , d ) after fracture. Scale bars 50 µm. ( e – h ) Representative immunohistochemical images of CD31-positive microvessels (arrowheads) within the callus tissue of wildtype ( e , f ) and Nlrp3 −/− mice ( g , h ) at 2 ( e , g ) and 5 weeks ( f , h ) after fracture. Scale bars: 50 µm. Histological analysis of TRAP-positive osteoclasts/HPF ( i ) and immunohistochemical analysis of CD31-positive microvessels/HPF ( j ) within callus tissue of wildtype (white bars, n = 8) and Nlrp3 −/− mice (black bars, n = 9–10) at 2 and 5 weeks after fracture (mean ± SEM). * p < 0.05 vs. wildtype.

Article Snippet: For the immunohistochemical detection of microvessels, the sections were stained with a monoclonal rat anti-mouse antibody against endothelial cell marker CD31 (1:100; Dianova, Hamburg, Germany).

Techniques: Immunohistochemical staining

Journal: Veterinary oncology (London, England)

Article Title: Intertumoral heterogeneity of the immune microenvironment in high grade canine mast cell tumors.

doi: 10.1186/s44356-025-00020-9

Figure Lengend Snippet: Fig. 3 Assessment of tumor infiltrating lymphocytes in canine MCTs. Correlative analyses of transcriptional T cell score with A. ICOSLG and B. PDCD1 transcripts (each dot represents an individual dog using log2 normalized expression of transcripts). Comparisons of C. transcriptional T cell score D. and density of CD3 + T cells determined immunohistochemically between low and high grade MCTs (mean and standard deviations displayed). Images of lymphoid aggregates consistent with tertiary lymphoid structures within the high grade MCT-12 using E. immunohistochemical staining against CD3 (DAB chromogen reaction and hematoxylin counterstain) and F. co-immunofluorescent labelling of CD3 (green), CD79b (red), KIT (white), nuclear DAPI (blue) labelling. (Scale bars = 200 μm. DAB- diaminobenzidine; DAPI- 4’,6’-diamidino-2-phenylinlindole. Correlative analyses performed using two-tailed Spearman correlation Spearman rho (rs) and p values displayed. Comparisons between two groups performed using two-tailed Mann–Whitney tests, ns = not significant.)

Article Snippet: A primary rat monoclonal antibody against CD3ε (CD3, Bio-Rad MCA1477T, clone CD312) was used at a concentration of 1/600 and incubated on the slides for 45 min at RT.

Techniques: Expressing, Immunohistochemical staining, Staining, Two Tailed Test, MANN-WHITNEY

Journal: Cell transplantation

Article Title: Human Placenta-Derived Mesenchymal Stem Cells Combined With Artificial Dermal Scaffold Enhance Wound Healing in a Tendon-Exposed Wound of a Rabbit Model.

doi: 10.1177/09636897241228922

Figure Lengend Snippet: Figure 8. Expression of CD31 and human nuclei. (A) Paraffin sections of skin lesions from autologous skin transplantation for 1 week were obtained, and the endothelial cells CD31 and human nuclei were stained by immunohistochemistry. Scale bars: 100 μm (upper row), 20 μm (lower row). (B) Quantification of CD31-positive expression. hPMSCs: human placenta-derived mesenchymal stem cells.

Article Snippet: After de-paraffinizing with xylene and rehydrating the sections (descending gradient of ethanol), primary antibodies against CD31 (1:1,000, OriGene, USA) or human nuclear antibody (1:500, Millipore, USA) were added at 4°C overnight, followed by incubation with horseradish peroxidaseconjugated affinipure goat anti-mouse immunoglobulin G (IgG) for 1 h at room temperature, followed by colorimetric detection using DAB (3,3′-diaminobenzidine) substrate, according to the manufacturer’s protocol20.

Techniques: Expressing, Transplantation Assay, Staining, Immunohistochemistry, Derivative Assay

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Generation of Connective Tissue-Free Microvascular Fragment Isolates from Subcutaneous Fat Tissue of Obese Mice

doi: 10.1007/s13770-023-00571-8

Figure Lengend Snippet: Histomorphology of subcutaneous adipose tissue from lean and obese mice. A , B HE-stained sections of the subcutaneous adipose tissue from a lean ( A ) and an obese ( B ) mouse. Scale bars: 30 µm. C , D Adipocyte diameter ( C , given in µm) and adipocyte density ( D , given in mm −2 ) within the subcutaneous adipose tissue from lean (white bars, n = 5) and obese (black bars, n = 5) mice. Means ± SEM. * p < 0.05 versus lean. E , F Sirius red-stained sections under polarized light, displaying larger blood vessels (arrows) with surrounding type I collagen within the subcutaneous adipose tissue from a lean ( E ) and an obese ( F ) mouse Scale bars: 40 µm. G Collagen fraction (given in % of adipose tissue) within the subcutaneous adipose tissue from lean (white bar, n = 5) and obese (black bar, n = 5) mice. Means ± SEM. * p < 0.05 vs. lean. H , I Immunohistochemical detection of CD31 + microvessels (arrows) between adipocytes (asterisks) within the subcutaneous adipose tissue from a lean ( H ) and an obese ( I ) mouse. Cell nuclei were stained with Hoechst 33342. Scale bars: 15 µm. J Microvessel density (given in mm −2 ) within the subcutaneous adipose tissue from lean (white bar, n = 5) and obese (black bar, n = 5) mice. Means ± SEM. * p < 0.05 versus lean

Article Snippet: For immunohistochemistry, sections were co-stained with a monoclonal rat anti-mouse antibody against CD31 (1:100; Dianova, Hamburg, Germany) and a polyclonal goat antibody against GFP (1:200; Rockland Immunochemicals, Limerick, PA, USA).

Techniques: Staining, Immunohistochemistry

Journal: Tissue Engineering and Regenerative Medicine

Article Title: Generation of Connective Tissue-Free Microvascular Fragment Isolates from Subcutaneous Fat Tissue of Obese Mice

doi: 10.1007/s13770-023-00571-8

Figure Lengend Snippet: Histomorphology of implanted MVF-seeded CGAG scaffolds. A , B HE-stained sections of CGAG scaffolds (borders marked by broken lines) seeded with MVF isolates from the subcutaneous adipose tissue of a lean ( A ) and an obese ( B ) mouse on day 14 after implantation into full-thickness skin defects within the dorsal skinfold chamber of recipient mice. Scale bars: 230 µm. C – E Sirius red-stained sections under polarized light of normal skin ( C ) as well as CGAG scaffolds seeded with MVF isolates from the subcutaneous adipose tissue of a lean ( D ) and an obese ( E ) mouse (arrows = collagen fibers). Scale bars: 12 µm. F Total collagen ratio (given as implant/skin) of CGAG scaffolds seeded with MVF isolates from the subcutaneous adipose tissue of lean (white bar, n = 10) and obese (black bar, n = 10) mice on day 14 after implantation into full-thickness skin defects within the dorsal skinfold chamber of recipient mice. Means ± SEM. G , H Immunohistochemical detection of CD31 + microvessels (arrows) within CGAG scaffolds seeded with MVF isolates from the subcutaneous adipose tissue of a lean ( G ) and an obese ( H ) mouse. Cell nuclei were stained with Hoechst 33342. Scale bars: 30 µm. I Microvessel density (given in mm −2 ) of CGAG scaffolds seeded with MVF isolates from the subcutaneous adipose tissue of lean (white bar, n = 10) and obese (black bar, n = 10) mice on day 14 after implantation into full-thickness skin defects within the dorsal skinfold chamber of recipient mice. Means ± SEM. * p < 0.05 versus lean. J – L Immunohistochemical detection of a CD31 + /GFP + microvessel (arrowhead) and a CD31 + /GFP − microvessel (arrow) within a CGAG scaffold seeded with an MVF isolate from the subcutaneous adipose tissue of a lean mouse. Cell nuclei were stained with Hoechst 33342. Scale bars: 20 µm. M CD31 + /GFP + microvessels (given in %) within CGAG scaffolds seeded with MVF isolates from the subcutaneous adipose tissue of lean (white bar, n = 10) and obese (black bar, n = 10) mice on day 14 after implantation into full-thickness skin defects within the dorsal skinfold chamber of recipient mice. Means ± SEM

Article Snippet: For immunohistochemistry, sections were co-stained with a monoclonal rat anti-mouse antibody against CD31 (1:100; Dianova, Hamburg, Germany) and a polyclonal goat antibody against GFP (1:200; Rockland Immunochemicals, Limerick, PA, USA).

Techniques: Staining, Immunohistochemistry

Journal: Cancer Biology & Medicine

Article Title: The inducible secreting TLR5 agonist, CBLB502, enhances the anti-tumor activity of CAR133-NK92 cells in colorectal cancer

doi: 10.20892/j.issn.2095-3941.2023.0033

Figure Lengend Snippet: Adoptively transferred CAR133-i502-NK92 cells successfully control tumor development in mice with CD133+ and CD133− mixed colon tumor models. (A) Schedule of the in vivo experimental design using CD133+ and CD133- mixed colon tumor model. (B) BLI illustrating colon cancer growth. (C) Kaplan–Meier survival curves generated from the survival of mice treated with CAR133-i502-NK92 and CAR133-NK92 cells. *** P < 0.001. (D) Expression of CD31 protein was tested in the mouse tumor tissue using IHC assays. Representative photomicrographs are shown (magnification ×400). The scale bar is 50 μm. *** P < 0.001. (E) Representative immunostaining images of CD3+ T-cell infiltration into cancer tissues. Representative staining of tumor sections in each experimental group is shown (magnification ×400). The scale bar represents 50 μm. *** P < 0.001.

Article Snippet: Tumor tissue sections were stained for infiltration by human T cells using a mouse monoclonal antibody against human CD3ε (Thermo Scientific, Waltham, MA, USA) and for tumor angiogenesis using a mouse monoclonal antibody against human CD31 (Abcam).

Techniques: Control, In Vivo, Generated, Expressing, Immunostaining, Staining